Comparación entre los tipos de proteinuria en una población pediátrica

Abstract

Proteinuria is an important piece of information for the diagnosis and follow up of renal disease.
Those ones of renal origin may be caused by defects in glomerular, tubular, mixed and myeloma
causes level. How to identify each one of these protein profiles is through the use of urinary protein
electrophoresis, a qualitative method which allows, by electrophoretic run, to separate the proteins
present in the urine. By changing run supports and the used dyes, sensitivity of this method may be
varied. The b2 microglobulin is a tubular injury marker protein. To compare the types of proteinuria
observed in uroproteinogramas performed in a pediatric population, using two different techniques: a)
agarose colloidal silver staining and; b) sodium dodecyl sulfate - agarose, and to evaluate which one
gives us more information about kidney damage. Evaluate if the measure of b2 microglobulin, confirm
the tubular proteinuria. Thirty-eight samples of 24 hours unconcentrated urine were analyzed, which
arrived during one year to the Central Llaboratory at Children’s Hospital “Dr Ricardo Gutierrez” with
order to urinary protein electrophoresis. Those ones were performed by using sodium dodecyl sulfate agarosa technique and agarose gel with colloidal silver staining. Commercial kits were used for both
methods. Along with the urinary protein electrophoresis, a micro particle enzyme immunoassay was
performed in 27 of the 38 received samples, for the assay of b2 microglobulin