Palabras clave
β-hidroxibutirato
ácidos grasos libres no esterificados
especificaciones técnicas
Cómo citar
Resumen
β-Hydroxybutyrate (βOH) and non-esterified free fatty acids (NEFA) are measured for the study of metabolic diseases and hypoglycemia, and βOH, in addition, for hyperglycemia and control in ketogenic diets. Verification should confirm that the performance declared by the manufacturer has been met. Objective: To verify the NEFA and βOH methods. Materials and methods: The CLSI guidelines EP15- A3 and EP6-A were followed. The Roche/Cobas 501 autoanalyzer was used. Results: NEFA repeatability conditions were as follows: level 1 (N1) coefficient of variation 0.60% and level 2 (N2) 0.62%. N1 βOH 1.54% and N2 0.56%. Intermediate precision: 2.51% N1 and 1.62% N2 for NEFA and 1.62% N1 and 0.78% N2 for βOH. Veracity: 0.7 mmol/L N1 and 1.6 mmol/L N2, verification ranges 0.55-0.66 mmol/L and 1.38- 1.53 m mol/L for NEFA; 0.3 mmol/L N1 and 2.9 mmol/L N2, verification ranges 0.294-0.325 mmol/L and 2.793-2.942 mmol/L for βOH. The veracity estimation bias was 11.85% and 11.98% for NEFA and 4.83% and 1.92% for βOH. Linearity was up to 2.4 mmol/L for NEFA and up to 3.23 mmol/l for βOH. Conclusions: It was possible to verify precision under repeatability and intermediate conditions for both analytes, veracity from a clinical point of view, although not statistical for NEFA, and clinical and statistical veracity for βOH. Statistical and clinical linearity was verified for NEFA and clinical linearity for βOH.
Referencias
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